The field of tissue engineering is finally making medical science-fiction a reality. I am a biomedical engineer that specializes in cell culture and tissue engineering. Developing living tissues has a high impact on the medical field; tissues and organs now produced can replace transplants from one patient to another. My name is Justin R. Papreck; if, after viewing this website, you would like to know any more information about me of my work, please contact me with the contact information on the last page. Thank you.
I spent nearly a year working in the Peptide Biology Laboratory of Dr. Wylie Vale as a Research Assistant for Dr. Elizabeth Flandreau. This position primarily entailed in situ hybridization (ISH) of central gene-expression changes from mice exposed to social stress, with densiometric analysis of the ISH. Gene expression analysis via quantitative real-time polymerase chain reaction (qPCR) tracked the changes from mice exposed to social stress followed by high fat diet. Other duties included tissue collection and processing including brain removal, sectioning (cryostat and microtome) for in situ hybridization or brain micro-dissection, RNA extraction, and production of cDNA for qPCR.
The research involved patch-clamping of cells and molecular biology. I conducted complex electrophysiological experiments using either primary carotid body glomus cells or transfected HeLa cells; electrophysiological assessment was characterized by patch clamp techniques and analyzed on the computer.
During my tenure as an undergraduate, I pursued a special contracted major joining physics and biology call"Biotechnology" in which I earned my Bachelor's of Science at Illinois Wesleyan University. I further conducted research in the Department of Biology working with Dr. Loni Walker on the moss Ceratodon purpureus. The study examined the effects of known plant hormones on the gravitropic response. I also spent one summer as an Intern for Motorola testing a new design for an implantable glucose sensor for diabetics.
I was a teaching assistant in the Department of Physics for two years, as well as a physics tutor for the introductory physics classes both with and without calculus.